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| Titel: |
Detection of Legionella pneumophila by PCR-ELISA Method in Industrial Cooling Tower Water |
| Auteur: |
Soheili Majid Nejadmoghaddam Mohammad Reza Babashamsi Mohammad Ghasemi Jamileh Jeddi Tehrani Mahmood |
| Verschenen in: |
Pakistan journal of biological sciences |
| Paginering: | Jaargang 10 (2007) nr. 22 pagina's 4015-4021 |
| Jaar: |
2007 |
| Inhoud: |
Water supply and Cooling Tower Water (CTW) are among the most common sources of Legionella pneumophila (LP) contamination. A nonradio active method is described to detect LP in industrial CTW samples. DNA was purified and amplified by nested -PCR with amplimers specific for the 16s rRNA gene of LP. The 5� end biotinylated oligomer probe was immobilized on sterptavidin B coated microtiter plates. The nested-PCR product was labeled with digoxigenin and then hybridized with 5�-biotinylated probes. The amplification products were detected by using proxidase-labled anti dioxygenin antibody in a colorimetric reaction. The assay detected LP present in 1 L of 5 CTW samples examined. All of the samples were Legionella positive in both culture and PCR-ELISA methods. The PCR-ELISA assay appears to exhibit high specificity and is a more rapid technique in comparison with bacterial culture method. Thus could prove suitable for use in the routine examination of industrial CTW contamination. |
| Uitgever: |
Asian Network for Scientific Information (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 5 van 39 gevonden artikelen
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