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                                       Details van artikel 4 van 35 gevonden artikelen
 
 
  Assessment of Genetic Diversity of Local and Exotic Brassica napus Germplasm
 
 
Titel: Assessment of Genetic Diversity of Local and Exotic Brassica napus Germplasm
Auteur: Mian Afaq Ahmad
Iqbal Munir
Waqar Ali
Zahoor Ahmad Swati
Muhammad Saeed Khattak
Quahir Sohail
Imran Khan
Verschenen in: Pakistan journal of biological sciences
Paginering: Jaargang 10 (2007) nr. 15 pagina's 2490-2494
Jaar: 2007
Inhoud: Estimation of genetic diversity of Brassica germplasm provides the basis for rapeseed/mustard genetic improvement. Studies were undertaken to estimate the genetic diversity of 30 lines of Brassica napus using Randomly Amplified Polymorphic DNA (RAPD) primers. A total of 30 B. napus genotypes of local and exotic origin were characterized using molecular markers. Four RAPD primers were used to estimate the genetic distances among the genotypes in all the possible combinations. The genetic diversity study revealed different levels of genetic polymorphism for RAPD primers GLA05, GLA07, GLA09 and GLA10, resulting in amplification of 5.7, 3.5, 3.1 and 5.4 scorable bands (loci) per genotype, respectively. Individual genetic distances observed among B. napus genotypes ranged from 6.5 to 51%. Bivariate data matrix was generated and genetic distances were calculated using Unweighted Pair Group of Arithmetic Mean (UPGMA) procedure. The UPGMA cluster analyses revealed maximum genetic dissimilarity for 8966-1 and 8969-1 genotypes, closely followed by Ganyou-5, 89127-1, 89111-2 and Mlep-048. It is recommended that among the thirty B. napus genotypes, genetically distinct lines pointed out in the present study, should be used in future breeding programs for improvement of Brassica napus.
Uitgever: Asian Network for Scientific Information (provided by DOAJ)
Bronbestand: Elektronische Wetenschappelijke Tijdschriften
 
 

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