Alterations in Plasma Lipid Peroxidation and Total Antioxidant Status During Storage of Blood
Titel:
Alterations in Plasma Lipid Peroxidation and Total Antioxidant Status During Storage of Blood
Auteur:
Abdoljalal Marjani
Verschenen in:
Pakistan journal of biological sciences
Paginering:
Jaargang 9 (2006) nr. 13 pagina's 2520-2523
Jaar:
2006
Inhoud:
The aim of the present study was designed to determine the alteration of plasma lipid peroxidation-(by measuring the level of MDA) and total antioxidant status in stored blood. This study is carried out to find out the quantitative alterations and the useful length of stored blood. The whole blood were taken from 10 donors. Red Blood Cells (RBC) were counted in whole blood. The levels of Potassium (P) and lactate dehydrogenate activity(LDH) were measured in plasma for determination of hemolysis. The levels of malondialdehyde (MDA) and Total Antioxidant Status (TAS) were studied in plasma for determination of lipid peroxidation and antioxidant system, respectively. The measurement were performed at the day 0, 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 and 35 of the storage. The levels of malondialdehyde and Potassium and lactate dehydrogenate activity increased (p<0.05) depending on storage time whereas Total Antioxidant Status (TAS) and Red Blood Cells decreased (p<0.05). The alterations of MDA, TAS, P, LDH and RBC in the measurement days were as follows: MDA, P and LDH significantly increased at the day 9, 5 and 5 whereas TAS and RBC decreased at the day 13 and 29, respectively. These results suggest that increased level of MDA and decreased level of TAS in the stored blood can not improve the vialibilty and longevity of RBC by increasing cell damage caused by free radicals at the day mentioned above. To improve the quality of stored blood is to supplement blood donors with antioxidants and vitamins at least one week before blood collection to keep and store the stored blood for longer time until using for transfusion. We conclude that increased level of MDA and decreased TAS in stored blood which can cause the beginning of hemolysis. It is therefore necessary to control these factors before blood transfusion.
Uitgever:
Asian Network for Scientific Information (provided by DOAJ)
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