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| Titel: |
A rapid method for isolation of high quality DNA from leaves of Gmelina arborea (Roxb) for molecular analysis |
| Auteur: |
Madhan Shankar S.R. Sugumaran G. Kalaiyarasu T. Niket Bubna |
| Verschenen in: |
International journal of biotechnology applications |
| Paginering: | Jaargang 1 (2009) nr. 2 pagina's 16-19 |
| Jaar: |
2009 |
| Inhoud: |
Procuring considerable quantity of DNA of nearly fair quality from trees is difficult because ofthe presence of metabolites and mucilaginous substances, which is found in excess in their leaves thatinterfere with the isolation procedures. Gmelina arborea, a fast growing species of the familyverbenaceae occurs naturally in tropical and sub tropical regions of Asia. Currently, though there are anumber of methods for isolation of DNA like the CTAB method, SDS Method etc., no single method wassuitable for obtaining fine quality DNA from the leaves of Gmelina arborea for further molecular studiesbecause of the presence of polyphenols and mucilaginous substances in the leaves. Herein we describea modified protocol based on Dellaporta et al. (1983) for isolating DNA from dried leaves of Gmelinaarborea, which is suitable for further molecular analysis. The protocol overcomes the problem of coprecipitationwith contaminating agents. The yield of the DNA was found to be 50μg from 0.2 g ofstarting material. The purity of the DNA was also checked by the absorbance ratios at 260nm and280nm respectively. The DNA thus obtained was used for RAPD and ISSR analysis. |
| Uitgever: |
Bioinfo Publications (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 3 van 8 gevonden artikelen
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