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Details van artikel 22 van 23 gevonden artikelen
| Titel: |
The extraction of fibroin protein from Bombyx mori silk cocoon: Optimization of process parameters |
| Auteur: |
Mahesh Kumar Sah Arvind Kumar Pramanik K. |
| Verschenen in: |
International journal of bioinformatics research |
| Paginering: | Jaargang 2 (2010) nr. 2 pagina's 33-41 |
| Jaar: |
2010 |
| Inhoud: |
Optimization of protein extraction using silk cocoon was carried out by the design of experiment(DOE) to obtain the response surface methodology ( RSM ). Box-Behnken rotatable design was used tocreate an experimental program to provide data to model the effects of various factors on protein extractionefficiency. The variables chosen were sodium carbonate concentration ( 1 X ), Lithum bromide concentration( 2 X ) and temperature ( 3 X ). The mathematical relationship between protein extraction efficiency and threesignificant independent variables can be approximated by a second order quadratic model. RSM wasused to describe the individual and interactive effects of three variables at three levels, combined accordingto a Box-Behnken Design. The coefficient of determination (2 R ) for the model is 0.9761. Probability value( P < 0.0001) demonstrates a very high significance for the regression model indicating that Box-BehnkenDesign can be applied to the protein extraction from silk cocoon, and it is an economical way of obtainingthe maximum amount of information with the fewest experiments. |
| Uitgever: |
Bioinfo Publications (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 22 van 23 gevonden artikelen
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