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| Titel: |
Development of Random Amplified Polymorphism DNA Markers Linked to CMV-B2 Resistance Gene in Melon |
| Auteur: |
BUDI SETIADI DARYONO KENJI WAKUI KEIKO T. NATSUAKI |
| Verschenen in: |
Hayati Journal of Biosciences |
| Paginering: | Jaargang 16 (2009) nr. 4 pagina's 142-146 |
| Jaar: |
2009 |
| Inhoud: |
Two random amplified polymorphic DNA (RAPD) markers linked to CMV-B2 resistance gene (Creb-2) in melon cultivar Yamatouri were cloned and sequenced to design sequence characterized amplified region (SCAR) markers for detection of CMV-B2 resistance gene (Creb-2) in melon. SCOPE14 derived from OPE-14 yielded a single DNA band at 541 bp, while SCAPB05 derived from APB-05, yielded a single DNA band at 1,046 bp, respectively. Segregation of SCOPE14 and SCAPB05 markers in bulk of F2 plants demonstrated that they were co-segregated with RAPD markers from which the SCAR markers were originated. Furthermore, results of SCAR analysis in diverse melons showed SCAPB05 primers obtained a single 1,046 bp linked to Creb-2 in resistant cultivars Sanuki-shirouri and Kohimeuri. However, SCOPE14 failed to detect Creb-2 in diverse melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to CMV-B2 by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers. |
| Uitgever: |
The Indonesian Biological Society & Bogor Agricultural University (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 29 van 125 gevonden artikelen
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