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Details for article 6 of 7 found articles
| Title: |
The nonenzymatic template-directed ligation of oligonucleotides |
| Author: |
A.V. Lutay E. L. Chernolovskaya M. A. Zenkova V. V. Vlasso |
| Appeared in: |
Biogeosciences discussions |
| Paging: | Volume 3 (2006) nr. 1 pages 1-21 |
| Year: |
2006 |
| Contents: |
The nonenzymatic template-directed ligation of oligonucleotides containing 2',3'-cyclic phosphate was investigated in the presence of divalent cations. Ligation of the oligonucleotides readily occurred in the presence of Mg<sup>2+, Mn<sup>2+, Co<sup>2+, Zn<sup>2+, Pb<sup>2+. Efficacy of the metal ion catalysts inversely correlated with pKa values of the metal-bound water molecules. The intermolecular transesterification reaction yielded at least 95% of 2',5'-phosphodiester bonds independently on the nature of the metal ion. Relatively high reaction yields (up to 15%) suggest, that RNA fragmentation to oligonucleotides with 2',3'-cyclic phosphates, followed by reactions of those oligonucleotides could provide a source of new RNA molecules under prebiotic conditions. |
| Publisher: |
Copernicus GmbH (provided by DOAJ) |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 6 of 7 found articles
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