Biological Control of Rhizoctonia solani, the Causal Agent of Rice Sheath Blight by Antagonistics Bacteria in Greenhouse and Field Conditions
Titel:
Biological Control of Rhizoctonia solani, the Causal Agent of Rice Sheath Blight by Antagonistics Bacteria in Greenhouse and Field Conditions
Auteur:
Mostapha Niknejad Kazempour
Verschenen in:
Plant pathology journal
Paginering:
Jaargang 3 (2004) nr. 2 pagina's 88-96
Jaar:
2004
Inhoud:
Pseudomonas fluorescens isolates that inhibited growth of Rhizoctonia solani Kühn, the rice sheath blight pathogen were collected the rhizosplane and surrounding soil of healthy and rice sheath blight disease in farming of the Guilan province, Iran. Two hundred eighty eight isolates tested and among them only antagonistic ability of 8 isolates were demonstrated by using the dual culture method. According to the results of biochemical and morphological trials all isolates were identified as P. fluorescens biovar 3. By determining the effects of volatile metabolites, secretion of extracellular and antibiotics of these isolates inhibited mycelial growth of R. solani in vitro. All P. fluorescens isolates produced siderophore on King`s medium B, inhibited the mycelial growth of the R. solani. Antagonistics isolates reduced the germination and cause the lysis of sclerotia of R. solani. In greenhouse conditions antagonistic isolates were used by seed coating, soil drenching and foliar spray. Statistical analysis of data indicated that there existed significant differences between seed, soil and plant treatments. All of the isolates in seed coating are more effective. In the field conditions foliar spray of isolate B41 mixed with benlate were applied. The disease intensity in B41 isolate for seed coating, soil drenching and seed coating + foliar spray were 10.5, 11.75 and 18.75%, respectively, while the control plants showed 52% disease intensity. These results suggest that the P. fluorescens isolates studied have an excellent potential to be used as biocontrol agents of R. solani in rice at the field conditions.
Uitgever:
Asian Network for Scientific Information (provided by DOAJ)
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