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| Titel: |
Establishment of Virus-Free Taro (Colocasia esulenta cv. Fenghuayunaitou) by Meristem-Tip Culture Combined with Thermotherapy |
| Auteur: |
Yongwei Li Cheng Xu Jishuang Chen |
| Verschenen in: |
Plant pathology journal |
| Paginering: | Jaargang 1 (2002) nr. 2 pagina's 40-43 |
| Jaar: |
2002 |
| Inhoud: |
Virus-free taro (Colocasia esculenta) was obtained by elimination of dasheen mosaic virus (DsMV) through thermo-therapy and meristem-tip culture. Shoot tips as explants of field taro (cv. Fenghuayunaitou) were cultured on MS basal salts medium suppled with 2.0 mg l-1 benzylamiopurinne (BA) and tips were proliferated and proliferated for production of new buds. When the rootless seedlings transferred on MS basal salts medium, plantlets with roots were obtained within 20 days. For detection of DsMV from tube-seedlings, a molecular probe of the genomic RNA of DsMV HZ isolate (DsMV-HZ) was constructed by RT-PCR, for the 3` end partial sequence and used for dot blotting hybridization with 32P probe. Analysis of nucleotides and deduced amino acid sequence between DsMV-HZ isolate with documented references presented 80-95% similarity among the coat protein region and 3` end untranslated region. About 10% seedlings obtained by meristem-tip culture were identified as DsMV-free seedlings in this experiment. |
| Uitgever: |
Asian Network for Scientific Information (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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