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| Titel: |
Morphological, Serological and Molecular Analyses of Anthracnose-Causing Agent on Banana Fruit |
| Auteur: |
Nataša Duduk Mirko Ivanović Bojan Duduk |
| Verschenen in: |
Pesticidi i Fitomedicina |
| Paginering: | Jaargang 24 (2009) nr. 4 pagina's 281-286 |
| Jaar: |
2009 |
| Inhoud: |
Two species of the genus Colletotrichum, C. musae and C. gloeosporoides, occur as infecting species of banana. The study focused on examining the etiology of anthracnose on banana fruits sold on the domestic market. An isolate was obtained from a deseased banana fruit on PDA medium, forming a white colony with intensive and uniformed growth. It was not possible to identify the isolated fungus based on its morphological characteristics. Positive serological reaction in an ELISA test with monoclonal antibodies for C. acutatum indicated an antigen site for the used monoclonal antibodies. Positive reaction when C. gloeosporioides-specific primers were applied indicated a similarity in the ITS sequence ofthe fungus and the examined isolate from banana fruit. Although there are no available data in literature that C. gloeosporioides-specific CgInt primer can be used for amplification of the phylogenetically related C. musae, our results do not exclude that the isolate could be C. musae. The host plant, symptoms observed and colony characteristics of the fungus isolated from the banana fruit mostly correspond to C. musae. Based on morphological, antigenand gentic characteristics, the isolate from banana was determined as Colletotrichum sp., while species identification of the anthracnose-causing agent on banana requires additional analysis. |
| Uitgever: |
Institute of Pesticides and Environmental Protection (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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