Control of oxidation and contamination of strawberry (Fragaria X ananassa Duch.) cultivated in vitro
Titel:
Control of oxidation and contamination of strawberry (Fragaria X ananassa Duch.) cultivated in vitro
Auteur:
María Claudia SÁNCHEZ CUEVAS José Luis SALAVERRÍA
Verschenen in:
Revista científica UDO agrícola
Paginering:
Jaargang 4 (2004) nr. 1 pagina's 21-26
Jaar:
2004
Inhoud:
Strawberry is cultivated all around the world, not only for its digestive and tonic properties, but because of the nutritionalvalue of its fruits, important source of folate, vitamin C, fiber, potassium, flavonoids, antocianidin, phytochemicals andantioxidants. Prior experiences with strawberry micropropagation indicate that vitroplants are more uniform, producehigher number of runners, have better survival in the field, and the fruit yield increases in 24% than plants propagated by thetraditional method. It is necessary to avoid the contamination with microorganisms in order to achieve success in theestablishment, incubation and manipulation of the tissue in vitro, since contaminants can destroy the explants, delay theirdevelopment by competing with them or generate changes in the culture medium that negatively affect their survival andgrowth. Contamination can come from the explant or can be introduced during the manipulation of plant tissue. In theinitial attempts to introduce strawberry plants to in vitro conditions, a high contamination by fungi and bacteria wasobserved, besides tissue blackening in some cultivars. In the present work, disinfection of the strawberry explants cv. Fresnowas done by means of immersion of the shoots in the plant crown in commercial chlorine (sodium hypoclorite, 5.25%) fordifferent time intervals (10, 20 and 30 min) and various chlorine concentrations (10, 20 and 30%). The best treatment was20% chlorine with a 20 min immersion time, which better reduced the contamination, allowing a greater survival of theexplants and a higher shoot formation. The treatment that reduced more efficiently tissue oxidation was cystein (4 g/l) withlight, which allowed 100% explant survival.
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