Details van artikel 109 van 185 gevonden artikelen
In vitro regeneration of Laelia anceps ssp. dawsonii
Titel:
In vitro regeneration of Laelia anceps ssp. dawsonii
Auteur:
Hilda E. LEE ESPINOSA Antonio LAGUNA CERDA Joaquin MURGUÍA GONZÁLEZ Pablo ELORZA MARTÍNEZ Lourdes IGLESIAS ANDREU Benjamín GARCÍA ROSAS Felipe A. BARREDO POOL Nancy SANTANA BUZZY
Verschenen in:
Revista científica UDO agrícola
Paginering:
Jaargang 7 (2007) nr. 1 pagina's 58-67
Jaar:
2007
Inhoud:
Seeds of Laelia anceps ssp. dawsonii were germinated in vitro, this is a wild endangered orchid, originated in México andMesoamerica, with a high ornamental potential. Murashige & Skoog (1962) culture media, supplemented with 1-naftalenaceticacid (ANA), 6-benzyl-amino-purine (BAP), Kinetin (Kin) and indol-3-acético (AIA) acid, 2 mg L-1 each one, wasoptimum for callus induction under 16/8 h photoperiod (20.2 μmol•m-2•s-1). Callus was subcultured every 45 days in thesame culture medium producing 524 somatic embryoids in average, at the end of the third subculture. Somatic embryoidsgerminated in plants with shoots and roots in the same culture medium, and were transferred to VW supplemented with 2mg L-1 BAP, 1 mg L-1 AIA and 0.2 % active charcoal to induce their develop. Three months later, plantlets wereacclimatized in a greenhouse, with 100 % survivence.
Uitgever:
Universidad de Oriente Press (provided by DOAJ)
Bronbestand:
Elektronische Wetenschappelijke Tijdschriften
Details van artikel 109 van 185 gevonden artikelen