GENETIC CHARACTERISATION OF ITALIAN CHICKEN BREEDS USING A PANEL OF TWENTY MICROSATELLITE MARKERS
Titel:
GENETIC CHARACTERISATION OF ITALIAN CHICKEN BREEDS USING A PANEL OF TWENTY MICROSATELLITE MARKERS
Auteur:
Enrico Zanetti Chiara Dalvit Massimo De Marchi Riccardo Dal Zotto Martino Cassandro
Verschenen in:
Poljoprivreda
Paginering:
Jaargang 13 (2007) nr. 1 pagina's 197-200
Jaar:
2007
Inhoud:
Genetic relationships among four Veneto native breeds of chickens were studied on the basis of microsatellites polymorphisms. A total of 190 DNA samples (45 Robusta Lionata, 43 Robusta Maculata, 45 Ermellinata di Rovigo, 45 Pèpoi) and a commercial broiler line (12 Golden Comet) were genotyped at 20microsatellite loci. The average number of alleles per locus was 5 and the expected heterozygosity resulted lower for the local breeds than for the commercial broiler line used as reference. The inbreeding coefficient showed a deficit of heterozygotes, highest for the Robusta Lionata breed. Nei’s standard genetic distances corrected for bias due to sampling of individuals (Da), based on allele frequencies, and Reynolds distances (DReynolds) were calculated among breeds. The Robusta Lionata and Robusta Maculata resulted very similar approving the same genetic origin. A Neighbor-Joining tree drawn from DReynolds Key-words: chicken, microsatellites, genetic distances, biodiversity, conservation.distances clustered threegroups, one including the Robusta Lionata and Robusta Maculata breeds, the second one formed by the Ermellinata di Rovigo and the Golden Comet commercial line and the third by the Pèpoi. The results showedthe genetic differences occurring between the local chicken breeds
Uitgever:
Faculty of Agriculture in Osijek (provided by DOAJ)