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| Titel: |
Protocorm wounding enhanced Agrobacteriummediated transformation of Hygrochilus parishii (Veitch & Rchb.f.) Pfitz |
| Auteur: |
Witaya Pimda Sumontip Bunnag |
| Verschenen in: |
Advances in agriculture & botanics |
| Paginering: | Jaargang 2 (2010) nr. 3 pagina's 205-212 |
| Jaar: |
2010 |
| Inhoud: |
This study was aimed to encourage the use of protocorm wounding as a substitute ofacetosyringone in an attempt to reduce cocultivation time and to increase possibility of successful genetictransformation of Hygrochilus parishii(Veitch & Rchb.f.) Pfitz. Agrobacterium tumefaciens strain LBA4404harboring the plasmid pBI121 was employed as a transgene carrier. The plasmid pBI121 contained β-glucuronidase (gus) and kanamycin resistance (nptII) genes, both of which were driven by the CaMV35Spromoter. It was found that wounded protocorms cocultivated with A. tumefaciens for 30 min andacetosyringone-treated protocorms cocultivated for 30 min exhibited high gene expressions of 75 and80%, respectively, while normal protocorms (control) showed gene expression of 60% when cocultivatedwith A. tumefaciens for 45 min. The presence of CaMV35S promoter and NOS terminator detected byPCR technique confirmed a successful T-DNA integration. This suggested that protocorm wounding couldbe used as a substitute of acetosyringone in enhancement of genetic modification. |
| Uitgever: |
Bioflux (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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