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| Titel: |
Genetic transformation of Vanda lilacina Teijsm. & Binnend. with a chitinase gene |
| Auteur: |
Witaya Pimda Sumontip Bunnag |
| Verschenen in: |
Advances in agriculture & botanics |
| Paginering: | Jaargang 2 (2010) nr. 1 pagina's 71-78 |
| Jaar: |
2010 |
| Inhoud: |
This research was aimed to improve plantlet regeneration and to establish the optimal conditionsfor transformation of <b>Vanda lilacina</b> Teijsm. and Binnend. using <b>Agrobacterium tumefaciens<i/></b> strain LBA4404(pCAMBIA 1305.1) harboring a chitinase gene as a fungal resistant one. It was found that seeds successfullygerminated, and developed into protocorms and plantlets on solid New Dogashima (ND) mediumsupplemented with 1 % (w/v) potato juice. Protocorm proliferation was successful when culturing theprotocorms on solid ND medium supplemented with 3 mg/l BA. For successful genetic modification, theprotocorms were cocultivated with <b>A. tumefaciens</b> for 45 min. Bacterial elimination and selection of putativetransformants were implemented using 250 mg/l cefotaxime and 10 mg/l hygromycin, respectively. GUSassay revealed 80% of the protocorms expressing <b>gus</b> gene activity. Availability of 35S and NOS detected byPCR analysis confirmed gene integration. |
| Uitgever: |
Bioflux (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 7 van 12 gevonden artikelen
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