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                                       Details for article 8 of 20 found articles
 
 
  Identification of animal species in heat treated muscle homogenates using the polymerase chain reaction
 
 
Title: Identification of animal species in heat treated muscle homogenates using the polymerase chain reaction
Author: Velebit Branko
Marković Brankica
Janković Vesna
Borović Branka
Vesković-Moračanin Slavica
Appeared in: Acta veterinaria
Paging: Volume 59 (2009) nr. 2-3 pages 303-308
Year: 2009
Contents: The possibility of identification of animal species in pasteurized and sterilized muscle homogenates was studied. Homogenates consisted of beef, pork and chicken meat, mixed in equal amounts. Nitrite salt and polyphosphates were also added. Heat treatment included pasteurization at temperatures of 80ºC, 90ºC and 100ºC, while sterilization was performed at temperatures of 110ºC and 121ºC, respectively. Animal mitochondrial DNA was isolated from the samples and the target gene was subsequently amplified by PCR. The presence of species-specific bands indicated respective animal species in homogenates. The results showed that it was possible to identify the most common domestic animal species in heat treated muscle homogenates.
Publisher: Fakultet veterinarske medicine (provided by DOAJ)
Source file: Elektronische Wetenschappelijke Tijdschriften
 
 

                             Details for article 8 of 20 found articles
 
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