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| Titel: |
The Supervirulence Plasmid pToK47 from Agrobacterium tumefaciens A281Improves Transformation Efficiency of Hevea brasiliensis |
| Auteur: |
P. Arokiaraj R. Leelawathy H.Y. Yeang |
| Verschenen in: |
American journal of biochemistry and biotechnology |
| Paginering: | Jaargang 5 (2009) nr. 3 pagina's 137-141 |
| Jaar: |
2009 |
| Inhoud: |
<P>Problem statement:The present study investigates the ability of the pTok47 supervirulenceplasmid form Agrobacterium tumefaciens A281 to enhance genetic transformation in Hevea brasiliensis. Approach: Hevea anther callus was transformed via Agrobacterium-mediated genetic transformationusing two strains of Agrobacterium (GV2260 and GV3850) harboring the human serum albumincDNA and the supervirulent plasmid pToK47 from Agrobacterium tumefaciens A281. Thetransformed callus was selected using kanamycin as the selection agent Results: The Agrobacteriumstrain GV2260 benefited from the presence of the supervirulence plasmid in giving a higher frequencyof 7.4% transformed callus, 344.8% embryogenesis and 11.6% plantlet production compared to thecorresponding strain on its own giving 0.9% transformed callus, 204.5% embryogenesis and 4.4%plantlet production. Similarly, Agrobacterium strain GV3850 conferred a higher frequency using thesupervirulent plasmid, resulting in 3.5% transformed callus, 138.5% embryogenesis and 3.5% plantletproduction compared to the corresponding strain on its own giving 0.7% transformed callus, 137.5%embryogenesis and 9.0% plantlet production. These findings were confirmed by the Wilcoxon SignedRank Test that compared the effectiveness of the supervirulence plasmid in increasing the rate ofgenetic transformation in the calli surviving in kanamycin growth medium for GV2260 (p<0.001) andfor GV3850 (p<0.05). Conclusion:This study showed that both Agrobacterium strains benefited fromthe presence of the supervirulence plasmid in giving a higher frequency of transformed callus,embryoids and plantlets. These results suggest that a highly virulent binary vector system might proveespecially useful in generating high frequency transformation of Hevea. </P> |
| Uitgever: |
Science Publications (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 7 van 7 gevonden artikelen
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