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| Titel: |
Bio-Safety Technology in Production of Bovine Herpesvirus Type 5 (BoHV-5) Using an Alternative Serum-Free Medium |
| Auteur: |
Tereza C. Cardoso, Heitor F. Ferrari, Maria Cecília R. Luvizotto and Clarice W. Arns |
| Verschenen in: |
American journal of biochemistry and biotechnology |
| Paginering: | Jaargang 3 (2007) nr. 3 pagina's 125-130 |
| Jaar: |
2007 |
| Inhoud: |
The detection and growth of bovine herpes virus type 5 (BoHV-5) was evaluated in three different media without foetal bovine serum or animal protein by infecting three different cell lines. The OPTI-PRO, VP-SFM and RPMI 1640 media were supplemented by L-glutamine, antibiotics added by 5µg of insulin-like growth factor (IGF-I) and used to adapt CER, MDBK and CRIB cells in a statistic continuous culture system. The results obtained by CRIB and MDBK cells adaptation showed steady growth after 10th passages in OPTI-PRO medium, 20th passages in VP-SFM medium and 30th passages in RPMI 1640 medium, respectively. The OPTI-PRO and RPMI 1640 media were not able to support CER growth, even supplemented by IGF-1 and present apoptotic cells at 72h post-infection. The CER cells seeded after adaptation by 10th passages in VP-SFM medium added by 5g/20ml of IGF-1 growth factor revealed the best virus titres compared to MDBK and CRIB cell lines. It was concluded that CER, MDBK and CRIB cells infected by BoHV-5 serotype could be used for laboratory diagnosis propagated on much safer culture system of high biotechnology advances. |
| Uitgever: |
Science Publications (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 2 van 12 gevonden artikelen
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