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                                       Details for article 31 of 92 found articles
 
 
  Genetic Relatedness Among Isolates of Acanthamoeba Based on RAPD Analysis
 
 
Title: Genetic Relatedness Among Isolates of Acanthamoeba Based on RAPD Analysis
Author: Siti Ruhaya Abdul Manaf
Shuhaimi Mustafa
Nakisah Mat Amin
Abdul Manaf Ali
Appeared in: Journal of applied sciences
Paging: Volume 6 (2006) nr. 1 pages 15-19
Year: 2006
Contents: Acanthamoeba is a pathogen frequently infecting brain, eyes, skin and lung of human and animal. Seven Acanthamoeba local isolates from various sources, two Acanthamoeba type strains and one strain of Hartmannella vermiformis were characterized for their genetic variability using randomly amplified polymorphic DNA (RAPD) technique using four different 10-mer oligonucleotides primers. Electrophoresis of the amplification products generated DNA bands ranging from approximately 0.25 to 7.50 kbp in size. A genetic relatedness among the isolates was examined using Dice similarity coefficient as the genetic distance measured between the strains of Acanthamoeba and H. vermiformis. Three distinct clusters could be separated at genetic distance of approximately 0.330.
Publisher: Asian Network for Scientific Information (provided by DOAJ)
Source file: Elektronische Wetenschappelijke Tijdschriften
 
 

                             Details for article 31 of 92 found articles
 
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