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| Titel: |
A new analytical technique in capillary electrophoresis: Studying the levels of nucleotides in human breastmilk |
| Auteur: |
Javier Cubero Javier Sanchez Cristina Sanchez David Narciso Carmen Barriga Ana Beatriz Rodrigez |
| Verschenen in: |
Journal of applied biomedicine |
| Paginering: | Jaargang 5 (2007) nr. 2 pagina's 85-90 |
| Jaar: |
2007 |
| Inhoud: |
The effect of nucleotides in the newborn is a determinant in this first stage of life, and their correctlevel in breastmilk is vital. We have designed a new method for the assay of nucleotides in milk bycapillary electrophoresis (CE) after acid hydrolysis. Breastmilk samples were collected from healthymothers (ages, 25–35 years) of one month lactation, and stored at -20°C. The duplicated sampleswere dissociated by acidic hydrolysis (HClO4) and the CE assay was performed in an uncoatedfused-silica capillary using an alkaline (borate) electrophoretic separation system.The method gave good recoveries of 5´-mononucleotides. Under the conditions used, the actualCE analysis time was less than 20 minutes. The physiologically and nutritionally importantnucleotides were detected at concentrations of 387 μg/100ml for UMP-5P, 385.3 μg/100ml forAMP-5P, 67 μg/100ml for CMP-5P, 172 μg/100ml for TMP-5P and 315 μg/100ml for GMP-5P.Nucleotides are a significant nutrient in infant growth, and capillary electrophoresis is a sensitiveand efficient tool for the assay of nucleotides with a purine or pyrimidine base in breastmilk. |
| Uitgever: |
University of South Bohemia (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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