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| Titel: |
The cultured primary hepatocyte and its application in toxicology |
| Auteur: |
Peter M. Eckl Nikolaus Bresgen |
| Verschenen in: |
Journal of applied biomedicine |
| Paginering: | Jaargang 1 (2003) nr. 3 pagina's 117-126 |
| Jaar: |
2003 |
| Inhoud: |
The liver is the main organ involved in the metabolism of xenobiotic (foreign) compounds. Theresponsible enzymatic systems are the cytochromes P450 (mixed function oxidases or phaseI reactions) and enzymes coupling larger water soluble groups to the substrate (phase II reactions).Especially during phase I reactions, highly reactive metabolites can be formed capable of interactingwith DNA and causing mutations. On the other hand reactive xenobiotics may be detoxified.Therefore, the primary parenchymal liver cell (hepatocyte) appears to be the optimal and mostreliable in vitro system for the determination of mutagenicity/genotoxicity. Since however, primaryhepatocytes are proliferatively quiescent, a culture system had to be developed allowing forproliferation enabling the determination of induced changes at the chromosomal level. This papersummarizes the special features of primary hepatocytes, the findings on in vitro proliferation andthe application of hepatocyte cultures for in vitro and ex vivo/in vitro toxicological testing. |
| Uitgever: |
University of South Bohemia (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 8 van 8 gevonden artikelen
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