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| Titel: |
Comparison of the reactions to stress produced by X-rays or electromagnetic fields (50Hz) and heat: induction of heat shock genes and cell cycle effects in human cells |
| Auteur: |
Sergey V. Tokalov Stefan Pieck Herwig O. Gutzeit |
| Verschenen in: |
Journal of applied biomedicine |
| Paginering: | Jaargang 1 (2003) nr. 2 pagina's 85-92 |
| Jaar: |
2003 |
| Inhoud: |
The effects of irradiation (200kV X-ray, 5 Gy), extremely low frequency electromagnetic fields(ELF-EMF, 50 Hz, 60±0.2 μT) and/or heat shock (HS, 41°C for 30 min) on the expression ofseveral heat shock genes (HSP27, HSP60, HSP70, HSP75, HSP78, HSP90) in human HL-60leukaemia cells was studied by RT-PCR. In addition, the effects of these stressors on cell cycleprogression in exponentially and asynchronously growing cell cultures were studied by flowcytometry. The dynamics of cell division during successive cell cycles was monitored byfluorescence-labelling of the cells with carboxyfluorescein succinimidyl ester (CFSE). Finally, thecell cycle distribution was studied by staining with propidium iodide (PI). With respect to HSP geneexpression the three stressors produced similar effects. The combination of stressors (ELF-EMF andHS or Radiation and HS) strongly induced transcription of the HSP70 gene above the level inducedby each stressor alone. The cell cycle analysis, however, revealed striking differences in the cellularresponse to each stressor. Of particular interest was the observed thermoprotective effect of ELFEMFin heat shocked cells, an effect that was not seen in cells which were exposed to X-rays in thepresence of thermal stress. |
| Uitgever: |
University of South Bohemia (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 3 van 7 gevonden artikelen
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