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| Titel: |
Cancer T47D Cells by Immunocytochemistry |
| Auteur: |
Z. Motahari M. Etebary E. Azizi |
| Verschenen in: |
International journal of pharmacology |
| Paginering: | Jaargang 1 (2005) nr. 2 pagina's 112-117 |
| Jaar: |
2005 |
| Inhoud: |
In the present study, resistant subline of human breast cancer T47D cells was isolated by stepwise exposure to increasing concentrations of TAM (10-8- 10-6 M). The subline that showed resistance to 10-6 M of TAM, named T47D/TAMR-6, was compared to the parental cells with respect to the growth characteristics, cross-resistance to Adriamycin (ADR) and P-gp expression. A significant increase in doubling time of the T47D/TAMR-6 cells was observed when compared to the parental T47D cells. This indicates a relatively slow growth rate pattern of the resistant cells. The T47D/TAMR-6 cells also showed cross-resistance to 1x10-8 M concentration of ADR. Immunocytochemical analysis using monoclonal antibody (C494; Dako-Denmark) against human P-gp and LSAB2 detection kit (Dako-Denmark) revealed a significant increase in P-gp level in resistant cells when compared to parental cells. Therefore, present results indicate that P-gp can play an important role in development of resistance to TAM and cross-resistance to ADR in the human breast cancer T47D cells. |
| Uitgever: |
Asian Network for Scientific Information (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 19 gevonden artikelen
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