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Details for article 7 of 9 found articles
| Title: |
Identification of peroxidase and tyrosinase in green coconut water |
| Author: |
Coelho , M. A. Z. Duarte , A. C. P. Leite , S. G. F. |
| Appeared in: |
Ciencia y tecnologia alimentaria |
| Paging: | Volume 3 (2002) nr. 5 pages 266-270 |
| Year: |
2002 |
| Contents: |
Green coconut water is a largely consumed beverage, but its lifetime is restricted to a couple of days due to enzyme presence. Therefore, the present paper aimed enzyme characterization by using chromatography and electrophoresis. Primarily, measurements of enzymatic activity were realized and it was obtained 0.3 U / mL for peroxidase activity and 5.0 U / mL for tyrosinase activity, using fresh sample of green coconut water. Fast Protein Liquid chromatography (FPLC) and SDS-PAGE were used to enzymatic characterization. Based on anionic chromatography was possible to verify that peroxidase and tyrosinase showed retention times equal to 0.30 min and 1.30 min, respectively,. On gel filtration, the following molecular weight values were determined: 49.2 KDa for peroxidase and 73.8 KDa for tyrosinase. Molecular weights were also obtained in SDS-PAGE and it was 44.63 KDa for peroxidase, but tyrosinase was not detected in the conditions employed. © 2002 Altaga. All rights reserved. |
| Publisher: |
Sociedad Mexicana de Nutrición y Tecnología de Alimentos (provided by DOAJ) |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 7 of 9 found articles
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