Somatic Embryogenesis in Phoenix dactylifera L: Effect of Exogenous Supply of Sucrose on Proteins, Sugars, Phenolics and Peroxidases Activities During the Embryogenic Cell Suspension Culture
Title:
Somatic Embryogenesis in Phoenix dactylifera L: Effect of Exogenous Supply of Sucrose on Proteins, Sugars, Phenolics and Peroxidases Activities During the Embryogenic Cell Suspension Culture
Author:
J. Zouine I. El Hadrami
Appeared in:
Biotechnology
Paging:
Volume 3 (2004) nr. 2 pages 114-118
Year:
2004
Contents:
The present study was conducted to determine the effect of three concentrations of usually used (1.5, 3 and 6%) of sucrose on the proliferation of date palm cell suspension culture. The medium containing 3% sucrose, leads to best results than 1.5 and 6% sucrose especially for JHL cultivar. In fact, when sucrose concentration increased from 1.5 to 3%, the embryogenic mass significantly increased from 2.15 to 14.6 g per flask and 8.7 to 10 g per flask, respectively for JHL and BSTN cultivars. The biochemical analysis showed that the maturation medium with 3% sucrose significantly increased the proteins (14.9 mg g-1 FW for JHL cultivar and 5 mg g-1 FW for BSTN cultivar) and sugars contents (22.5 and 22.7 mg g-1 FW, respectively, for JHL and BSTN cultivars) in the embryogenic mass. In addition, as compared to 1.5 and 6%, the use of 3% sucrose enhanced phenolics contents (0.69 mg g-1FW) and peroxidases activities (2.9.103 n Kat g-1FW) in BSTN cultivar.
Publisher:
Asian Network for Scientific Information (provided by DOAJ)
Journal description