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| Titel: |
Effect of Media, Growth Regulators and Genotypes on Callus Induction and Regeneration in Wheat (Triticum estivum) |
| Auteur: |
Hamid Rashid Rizwana Abdul Ghani Zubeda Chaudhry |
| Verschenen in: |
Biotechnology |
| Paginering: | Jaargang 1 (2002) nr. 1 pagina's 49-54 |
| Jaar: |
2002 |
| Inhoud: |
Effects of media, growth regulators and genotype were investigated for callus induction, maintenance and regeneration in wheat (Triticum aestivum L. cvs., Chakwal 86, Rawal 87). The percentage of callus induction in Rawal 87 was 49.58 -75.62% on Murashige and Skoog (MS) medium and 58.31-91.58% on N6 medium. For Chakwal-86, callus induction frequency ranged from 52.08- 81.04% on MS medium and 53.12- 81.02% on N6 medium supplemented with 2mg/l 2, 4 dichlorophenoxy acetic acid(2,4-D) singly, and with three concentrations (0.1, 0.5 and 1mg/L) of 6-Benzylaminopurine (BAP). BAP proved to play no role in callus induction. Sorbitol promoted callus proliferation, on N6 medium supplemented with 2mg/L 2,4-D. For plant regeneration from calli, MS medium with 0.1mg/L IAA and four concentrations (0.5, 1.0, 2.5 and 5mg/L) of BAP were tested. Regeneration frequency was greater (31.9%) in Rawal 87 on the medium containing 0.5mg/L BAP whereas 2.5mg/L of BAP were found suitable for regeneration in Chakwal 86 (15.27%). |
| Uitgever: |
Asian Network for Scientific Information, Pakistan (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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