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| Titel: |
The development of strategy for obtaining single-chain recombinant antibodies against cell-surface biomarkers on the example of human CD34 |
| Auteur: |
Nikolaiev Iu. S. Gorbatiuk O. B. Tsapenko M. V. |
| Verschenen in: |
Biopolymers and cell |
| Paginering: | Jaargang 26 (2010) nr. 6 pagina's 492-498 |
| Jaar: |
2010 |
| Inhoud: |
Antibodies against cell-surface proteins play an important role in cell detection, separation and determination of differentiation stage. The globular structure of extracellular region of cell-surface antigens is frequently characterized by heavily glycosilation and/or is stabilized by disulphide bonds. In this case the obtaining of antibodies against such proteins is a substantive problem. Aim. The development of strategy for obtaining recombinant antibodies against cell-surface biomarkers. Methods. The research strategy is based on the construction of cDNA library of VH and VL genes of animals, immunized with recombinant antigen, and subsequent cell-based biopanning for the library enrichment with desired phage clones. High-throughput automated systems were used for the detection and isolation of antigen-specific clones. Results. We have obtained a panel of five antibodies that recognize an antigen on CD34+ cell surface using the methods of immunocytochemistry and flow cytometry. Conclusions. The proposed strategy may be used for obtaining antibodies against cell-surface antigens |
| Uitgever: |
Institute of molecular biology and genetics, National Academy of Sciences of Ukraine (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 10 van 10 gevonden artikelen
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