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| Titel: |
Newly synthesized carbocyanine fluorescent probes, their characteristics and behavior in proliferating cultures |
| Auteur: |
Goncharuk E. I. Borovoy I. A. Pavlovich E. V. Malyukin Yu. V. Grischenko V. I. |
| Verschenen in: |
Biopolymers and cell |
| Paginering: | Jaargang 25 (2009) nr. 6 pagina's 484-490 |
| Jaar: |
2009 |
| Inhoud: |
Aim. To study possibile application of C2, C9, C18 and JC-1 carbocyanine fluorescent dyes for cell culture characterization. Methods. Morphological methods, fluorescence-activated cell sorting (FACS) analysis, luminescent microscopy were used. Results. The studied carbocyanine probes were shown to be preserved in dividing cells for at least 4 duplications. It was found that carbocyanine probe JC-1 did not transit from cell to cell under combined culturing of labeled and non-labeled cells. Conclusions. The paper covers the use of carbocyanine fluorescent probes for long-term culturing of cell lines. Probes C9 and JC-1 were optimal for the proliferative culture observation, allowing to trace mitochondrial functional state |
| Uitgever: |
Institute of molecular biology and genetics, National Academy of Sciences of Ukraine (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 8 van 11 gevonden artikelen
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