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| Titel: |
Construction of bifunctional molecules specific to antigen and antibody’s Fc-fragment by fusion of scFv-antibodies with staphylococcal protein A |
| Auteur: |
Oliinyk O. S. Kaberniuk A. A. Redchuk T. A. Kolibo D. V. Komisarenko S. V. |
| Verschenen in: |
Biopolymers and cell |
| Paginering: | Jaargang 25 (2009) nr. 3 pagina's 245-249 |
| Jaar: |
2009 |
| Inhoud: |
Aim. To develop approach for detection of scFv and their complexes with antigens. Methods. The fusion proteins, which include sequences of scFv and staphylococcal protein A, were constructed and the obtained bifunctional molecules were immunochemically analysed. Results. It was shown, that scFv fused with protein A and their complexes with antigens are effectively recognized by labelled immunoglobulins with unrestricted antigenic specificity. Conclusions. The fusion of scFv with protein A fragment is a perspective approach to increase the efficiency of application in ELISA. The obtained scFv, fused with protein A, could be used for development of test-systems for the detection of diphtheria toxin. |
| Uitgever: |
Institute of molecular biology and genetics, National Academy of Sciences of Ukraine (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 7 van 20 gevonden artikelen
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