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| Titel: |
Effect of 24-epibrassinolide on lipoxygenase activity in maize seedlings under cold stress |
| Auteur: |
Kopich V. N. Kretynin S. V. Kharchenko O. V. Litvinovskaya R. P. Chashina N. M. Khripach V. A. |
| Verschenen in: |
Biopolymers and cell |
| Paginering: | Jaargang 26 (2010) nr. 3 pagina's 218-224 |
| Jaar: |
2010 |
| Inhoud: |
Aim. To investigate 24-epibrassinolide influence on the maize seedlings 9- and 13-lipoxygenases activity (9- and 13-LOX) under normal conditions (25o C) and cold stress (5o C). Methods. LOX activity was measured after treatment of seedlings with 0.01 and 1 ìM 24-epibrassinolide. The enzymes were extracted from maize seedlings with 0.1 M sodium acetate (pH 4.5) buffer, supplemented with a non-ionic detergent (0.1 % Brij-99) and EDTA (0.1 mM). The 9- and 13-LOX activities were determined spectrophotometrically at 234 nm using linoleic acid as substrate at pH 6.0 and 7.0 in the presence or absence of 0.02 % Lubrol PX. Results. 3-6-fold increase in LOX activity of 24-epibrassinolide-treated seedlings was demonstrated under normal conditions. Cold stress in the presence of 1µM 24-epibrassinolide enhances the activities of 9- and 13-LOX by 4 and 10 times, respectively. Conclusions. The results obtained enlarge our understanding of possible pathways of LOX metabolites involvement in the formation of cell response to brassinosteroids. |
| Uitgever: |
Institute of molecular biology and genetics, National Academy of Sciences of Ukraine (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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