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Details van artikel 38 van 65 gevonden artikelen
| Titel: |
OPTIMIZING IMMUNOENZYMATIC REACTIONS (ELISA) FOR THE DETECTION OF ANTIBODY AGAINST NDV VIRUS |
| Auteur: |
Daniela Botus Tatiana Oncescu |
| Verschenen in: |
Analele Universitatii Bucuresti. Chimie |
| Paginering: | Jaargang XV (2006) nr. II pagina's 33-41 |
| Jaar: |
2006 |
| Inhoud: |
The aim of this paper is to develop an ELISA diagnostic kit for antibody detection against NDV virus and to optimize the involved reactions. Therefore, we selected the optimal concentrations of coated antigen, antibodies (from sera) and peroxidase labeled conjugate. Our choice was made in agreement with ELISA technique requirements, which state that the absorbance for positive control serum should be higher than 1.300 and for the negative control serum lower than 0.300. We chose the incubation times for antigen (over night, 4 C), sera and conjugate (30 minutes, 37 C) and for substrate-choromogen mixture (10 minutes). Taking into account these data we elaborated a kit very useful for diseases control and diagnostic. |
| Uitgever: |
Ars Docendi Publishing House (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 38 van 65 gevonden artikelen
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