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                                       Details for article 41 of 83 found articles
 
 
  KINETIC STUDY OF THE pH INFLUENCE ON BSA THERMAL DENATURATION
 
 
Title: KINETIC STUDY OF THE pH INFLUENCE ON BSA THERMAL DENATURATION
Author: V.E. Sahini
G. Csire
C. Popa-Tilimpea
Alina Tirsoaga
Appeared in: Analele Universitatii Bucuresti. Chimie
Paging: Volume XI (2002) nr. I pages 127-132
Year: 2002
Contents: The kinetics of BSA thermal denaturation was studied in buffer solutions at pH=2; 7.8 and 10using a dilatometric method. In order to compare the rate constant values (k) of this process, theplot of V versus temperature was used in order to obtain k (considering some approximations)at a given temperature:A aVk! " . In this equation, V is the volume difference between theBSA solution and the buffer used as a solvent, A is the area of the V = f(T) plot obtained for agiven step of the considered thermal denaturation process and a is the part of this surfacecorresponding to a given temperature T. In this study some conformational changes induced bythermal denaturation of BSA macromolecule were evidenced, the temperatures of transition aswell as the G# values for the first transition being presented and discussed.
Publisher: Ars Docendi Publishing House (provided by DOAJ)
Source file: Elektronische Wetenschappelijke Tijdschriften
 
 

                             Details for article 41 of 83 found articles
 
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