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Details for article 47 of 143 found articles
| Title: |
DNA-BASED BIOSENSOR FOR DETECTION OF GENETICALLY-MODIFIED ORGANISMS |
| Author: |
A.G. Nica M. Mascini A.A. Ciucu |
| Appeared in: |
Analele Universitatii Bucuresti. Chimie |
| Paging: | Volume XIII (2004) nr. I-II pages 85-94 |
| Year: |
2004 |
| Contents: |
An electrochemical genosensor for the monitoring of hybridization was used in orderto develop and characterize a DNA biosensor-based assay for the detection of geneticallymodifiedorganisms (GMOs). Screen-printed gold electrodes were modified with a suitable thioltetheredDNA sequence (probe) related to the sequence of the 35S promoter (target) inserted inthe genome of GMOs and responsible for regulating the transgene expression. An enzymeamplifieddetection scheme was applied in order to quantify the electrochemical signal producedby reaction between probe sequence and target sequence (analyte). The assay was firstlycharacterized using synthetic oligonucleotides. Relevant parameters such as probe concentration,hybridization and enzymatic reaction time were investigated and optimized. Electrochemicaltechniques for DNA-modified electrodes control were used. |
| Publisher: |
Ars Docendi Publishing House (provided by DOAJ) |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 47 of 143 found articles
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