|
| << previous | next >> |
Journal description
All volumes of the corresponding journal
All issues of the corresponding volume
All articles of the corresponding issues
Details for article 50 of 143 found articles
| Title: |
Electrochemical DNA based biosensor for the detection of genotoxic compounds in fish bile Samples |
| Author: |
A.M. Tencaliec G. Bagni M. Mascini V. Magearu |
| Appeared in: |
Analele Universitatii Bucuresti. Chimie |
| Paging: | Volume XIV (2005) nr. I-II pages 179-186 |
| Year: |
2005 |
| Contents: |
A disposable electrochemical DNA based biosensor for the detection of genotoxiccompounds in fish bile samples as marker of PAHs (polycyclic aromatic hydrocarbons)exposured at contaminated sites has been reported. The DNA biosensor is assembled byimmobilising double stranded Calf Thymus DNA onto the surface of a disposable carbon screenprintedelectrode. The oxidation signal of the guanine base, obtained by a square wavevoltammetric scan, is used as analytical signal to detect the DNA damage; the presence of lowmolecular weight compounds with affinity for nucleic acids is measured by their effect on theguanine oxidation peak. Preliminary analysis of some PAH metabolites standard solutions wereperformed, in order to establish their behaviour with DNA based biosensor. Also, in order tohighlight the differences between the not exposed bile and the injected bile, a deconjugation (orhydrolisis) process of the bile has been performed.The applicability of such a biosensor foranalysing bile samples of fish from sites with high ecological risk was evaluated. |
| Publisher: |
Ars Docendi Publishing House (provided by DOAJ) |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 50 of 143 found articles
| << previous | next >> |