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Details van artikel 41 van 83 gevonden artikelen
| Titel: |
KINETIC STUDY OF THE pH INFLUENCE ON BSA THERMAL DENATURATION |
| Auteur: |
V.E. Sahini G. Csire C. Popa-Tilimpea Alina Tirsoaga |
| Verschenen in: |
Analele Universitatii Bucuresti. Chimie |
| Paginering: | Jaargang XI (2002) nr. I pagina's 127-132 |
| Jaar: |
2002 |
| Inhoud: |
The kinetics of BSA thermal denaturation was studied in buffer solutions at pH=2; 7.8 and 10using a dilatometric method. In order to compare the rate constant values (k) of this process, theplot of V versus temperature was used in order to obtain k (considering some approximations)at a given temperature:A aVk! " . In this equation, V is the volume difference between theBSA solution and the buffer used as a solvent, A is the area of the V = f(T) plot obtained for agiven step of the considered thermal denaturation process and a is the part of this surfacecorresponding to a given temperature T. In this study some conformational changes induced bythermal denaturation of BSA macromolecule were evidenced, the temperatures of transition aswell as the G# values for the first transition being presented and discussed. |
| Uitgever: |
Ars Docendi Publishing House (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 41 van 83 gevonden artikelen
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