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| Titel: |
COMPARISON BETWEEN NYLON-BASED AND NITROCELLULOSE-BASED POTENTIOMETRIC BIOSENSORS IN GLUCOSE ASSESSMENT |
| Auteur: |
Aurelia-Magdalena Pisoschi A.F. Danet |
| Verschenen in: |
Analele Universitatii Bucuresti. Chimie |
| Paginering: | Jaargang XV (2006) nr. I pagina's 39-44 |
| Jaar: |
2006 |
| Inhoud: |
This paper aims at comparing the analytical performances in glucose potentiometric determination by means of nitrocellulose-based and nylon-based enzyme electrodes. Two glucose oxidase-based enzyme–electrodes were obtained by entrapping the enzyme within two different semipermeable membranes: nylon (Biodyne B) and nitrocellulose (Biotrace NT). The obtained enzyme membranes were immobilized, consecutively, on the sensitive bulb of the glass electrode. The pH variation in time was monitored for both enzyme electrodes, at different glucose concentrations; the more concentrated the solution, the greater the pH decrease, because the amount of gluconic acid generated in the enzyme-catalysed reaction is greater. Calibration graphs were obtained for both enzyme electrodes; an approximate linear range was obtained between 10-4M and 10-3M; the analytical signal obtained with the nylon-based enzyme electrodes was greater than the one obtained with the nitrocellulose-based enzyme electrodes, at the same enzyme loading and the same glucose concentration. |
| Uitgever: |
Ars Docendi Publishing House (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 14 van 83 gevonden artikelen
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