|
| << vorige | volgende >> |
Tijdschrift beschrijving
Alle jaargangen van het bijbehorende tijdschrift
Alle afleveringen van het bijbehorende jaargang
Alle artikelen van de bijbehorende aflevering
Details van artikel 13 van 14 gevonden artikelen
| Titel: |
Ultrastructural Evidences of Hepatitis B Infection in Human Liver Biopsies Disclose Complex Assembly and Morphogenesis Pathways for Hepatitis B Virus |
| Auteur: |
Viviana Falcon Ivon Menéndez Nelson Acosta-Rivero Mineko Shibayama Marıa-C de la Rosa Jose Luna- Munoz Magdalena Miranda-Sanchez Jorge V. Gavilondo Deliana Lopez Santiago Duenas-Carrera Bienvenido Gra Glay Chinea Luisa Tamayo Garcia Waldo Garcia Eduardo Vidal Enrique Arus-Soler Jose Silva Felix Alvarez Emilio F. Acosta Jesus Seoane Juan Morales-Grillo Eduardo Penton Juan Kouri Victor Tsutsumi |
| Verschenen in: |
American journal of infectious diseases |
| Paginering: | Jaargang 4 (2008) nr. 2 pagina's 96-103 |
| Jaar: |
2008 |
| Inhoud: |
Despite of recent advances on acknowledge of hepatitis B virus (HBV) structure and biology, little is known about the morphogenesis and release of virions. In this study, the ultrastructural analysis of HBV components in liver biopsies from chronically HBV-infected patients disclosed complex assembly and morphogenesis pathways for HBV. HBV core (HBcAg) and surface (HBsAg) antigens were specifically identified in all liver biopsies from HVB-infected patients. HBcAgcontaining core-like particles 24-28 nm in diameter were observed both in nucleus and cytoplasm of hepatocytes. Dane’s-like particles were detected either budding to or into the lumen of ER close toHBsAg containing tubular structures. Besides, Dane’s-like particles were detected in different vesicular compartments resembling multivesicular endosomes. Other kind of enveloped HBV-like particles similar to the previously described cobra-shaped and horn-shaped particles were also observed in hepatocytes. Some of these particles were connected to the vesicle membrane through a stalk 20-22 nm in diameter. On the other hand, spherical subviral particles (SVP) were frequently observed in cytoplasmic vesicles. Moreover, a minor proportion of enveloped HBV-like particles budding through the plasma membrane to the extracellular space and bile canaliculi were detected.Interestingly, Dane’s-like particles in the bile canaliculi and entering into ductular-like cells were shown. Strikingly, Dane’s-like particles close to tubular structures and specific immunolabeling forHBcAg both in cytoplasm and nuclei of stellate-like cells were detected. Remarkably, HVB-like particles appeared entering hepatocytes from large cytoplasmic processes of fibrocytes raising theinteresting possibility of a cell to cell passage of HBV through direct transcellular migration. |
| Uitgever: |
Science Publications (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 13 van 14 gevonden artikelen
| << vorige | volgende >> |