Isolation and Identification of Vancomycin Resistant Staphylococcus aureus from Post Operative Pus Sample
Titel:
Isolation and Identification of Vancomycin Resistant Staphylococcus aureus from Post Operative Pus Sample
Auteur:
Subhankari Prasad Chakraborty1, Santanu KarMahapatra1, Manjusri Bal2 and Somenath Roy1*
Verschenen in:
Al Ameen journal of medical sciences
Paginering:
Jaargang 04 (2011) nr. 02 pagina's 152-168
Jaar:
2011
Inhoud:
Staphylococcus aureus is most frequently isolated pathogen causing bloodstream infections, skin and soft tissue infections and pneumonia. Recently, S. aureus have evolved resistance to both synthetic and traditional antibiotics. This study was carried out to isolate pathogenic S. aureus from post-operative pus sample, and VRSA was identified by evaluation of resistance patterns using conventional antibiotics. Thirty post operative pus samples were collected from nearby Hospital and species identification was confirmed by Gram staining, standard biochemical tests and PCR amplification of the nuc gene. Antibiotic susceptibility tests were carried out by MIC, MBC, DAD test and BHI vancomycin screening agar. VRSA were confirmed by PCR amplification of the vanA and vanB genes. From this study, it was observed that isolated S. aureus strains are pathogenic; 30% of strains were resistant to penicillin G, ampicillin and erythromycin; 26.67% strains were resistant to cephotaxime, gentamycin, streptomycin, tetracycline, chloramphenicol, norfloxacin, methicillin and vancomycin.
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