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Details for article 2 of 11 found articles
| Title: |
Aldosterone-dependent Generation of Tubules Derived from Renal Stem/Progenitor Cells |
| Author: |
W W. Minuth L. Denk |
| Appeared in: |
Transplantationsmedizin |
| Paging: | Volume 20 (2008) nr. 1 pages 42-47 |
| Year: |
2008 |
| Contents: |
Considerations about the application of stem/progenitor cells in renal injury and repair are the subject of intense biomedical and clinical research. In recent experiments it was shown that aldosterone exhibits a tubulogenic activity on renal stem/progenitor cells. However, little information is available about the signaling between the steroid hormone, the mineralocorticoid receptor (MR) including the interacting proteins in the cytoplasm of the target cell. To obtain new insights in the molecular signalling of MR embryonic tissue from neonatal rabbit kidney containing renal stem/progenitor cells was kept at the interface of polyester fleeces creating an artificial interstitium. Culture was performed in a perfusion container with always fresh and chemically defined Iscove’s Modified Dulbecco’s Medium (IMDM) for 13 days. The development of tissue was controlled on cryosections labeled with fluorescent Soybean Agglutinin (SBA) and other cellbiological markers. Fluorescence microscopy revealed that cultures without hormone application failed to generate SBA-labeled tubules, while treatment with aldosterone (1 x 10-7 M) resulted in development of numerous tubules showing intensive reaction with SBA. The tubulogenic action of aldosterone depends on the applied hormone concentration and is inhibited by the application of spironolactone and canrenoate. Interference in the molecular contact between the mineralocorticoid receptor (MR) and heat shock protein (hsp) 90 using geldanamycin or radicicol results in a lack of tubule development. Disturbing the contact between MR and hsp 70 with quercetin and KNK 437 shows only to a minimal degree structured tubules. Preventing the interaction between MR and immunophilins by cyclosporin A and rapamycin does not influence the generation of numerous SBA-labeled tubules, while administration of FK 506 demonstrates a lack of tubule development. In conclusion, the actual results suggest that the signal for structural development of tubules induced by aldosterone is mediated by interaction of MR, hsp 90, hsp 70 and especially immunophilin FKBP 12. |
| Publisher: |
Pabst Science Publishers |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 2 of 11 found articles
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