The electrochemically synthesized Polypyrrole-Polyvinyl Sulphonate (Ppy-PVS), Poly(N-methylpyrrole) - Polyvinyl Sulphonate (P(NMP)-PVS) and their co-polymer Polypyrrole- Poly(N-methylpyrrole)-Polyvinyl Sulphonate (Ppy-P(NMP)-PVS) films on indium tin oxide (ITO) coated glass electrode have been investigated by galvanostatic method. This study reveals that as compared to (P(NMP)-PVS) and (Ppy-P(NMP)-PVS), the Ppy-PVS film provide a polymer matrix with very good porosity, mechanical and environmental stability, uniform surface morphology and higher conductivity, which is suitable for the immobilization of biocomponent. The synthesized films were characterized using electrochemical technique, electrical conductivity, Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).