Incorporation of the glucose in presence of phosphate and acetate buffer were studied to investigate the poly(N-methylpyrrole)/polyvinyl sulfonic acid/sodium nitrate/glucose oxidase (P(NMP)/PVS/NaNO3/GODx) electrode. The P(NMP)/PVS/NaNO3 films were electrochemically synthesized and characterized by using electrochemical technique, FTIR and SEM. Glucose oxidase was immobilized by cross-linking via glutaraldehyde on the galvanostatically synthesized poly(N-methylpyrrole) /polyvinyl sulfonic acid/sodium nitrate (P(NMP)/PVS/NaNO3) film. The higher sensitivity of P(NMP)/PVS/NaNO3/GODx electrode was recorded in phosphate buffer than that of acetate buffer and that was found to be 4 µA/mM and 2.2 µA/mM, respectively. The kinetics parameters of P(NMP)/PVS/NaNO3/GODx electrode were determined for the phosphate buffer and acetate buffer. In phosphate buffer the observed values of Km and Imax were 14.4 mM and 111.2 mA, respectively and in acetate buffer 16.9 mM and 68 mA, respectively. The stability of synthesized P(NMP)/PVS/NaNO3/GODx electrode in phosphate buffer was found to be more than that of acetate buffer.
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