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                                       Details van artikel 2 van 4 gevonden artikelen
 
 
  Assessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassays
 
 
Titel: Assessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassays
Auteur: Anabel Bocanegra Alonso
Gildardo Rivera Sánchez
Jesús Rivas Espinosa
Rosa Issel Acosta González
Virgilio Bocanegra García
Ángel Trigos Landa
Verschenen in: Bioquimia
Paginering: Jaargang 31 (2006) nr. 4 pagina's 140-145
Jaar: 2006
Inhoud: Hyperprolactinemia leads to several diagnoses, however, the immunoassay used for the prolactin determination and the presence of macroprolactin, could induce a misdiagnosis. The aim of this study was to evaluate the usefulness of separation and detection of macroprolactin by precipitation with polyethylene glycol, using two commercial immunoassays, Axsym Abbott and Advia Centaur Bayer in patients with established diagnosis of hyperprolactinemia. One hundred and seventeen samples were tested. Samples were obtained from female patients aged between 22 to 59 years old. We determined total prolactin (PRL), macroprolactin and free prolactin. In 30 of these samples hyperprolactinemia was detected. PRL recovering percentages of supernatant fraction were from 6.5% to 78.5% and from 26.2% to 75.4% when we compared Advia to Axsym systems initial values, respectively. Based on the PRL recovering percentage, 7 samples with significant macro-PRL presence were observed. The macroprolactin prevalence in these 30 samples, after PEG precipitation, was of 23% and 10% in the Axsym and Advia system respectively. Based upon these findings, we establish that references values need to be determined for both instruments, having applied the polyethylene glycol precipitation for the prolactine determination.
Uitgever: Asociación Mexicana de Bioquímica Clínica, A.C.
Bronbestand: Elektronische Wetenschappelijke Tijdschriften
 
 

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