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| Titel: |
Screening for the coccidiostats halofuginone and nicarbazin in egg and chicken muscle: development of an ELISA |
| Auteur: |
Huet, A. -C. Mortier, L. Daeseleire, E. Fodey, T. Elliott, C. Delahaut, P. |
| Verschenen in: |
Food additives and contaminants. Pt. A, Chemistry, analysis, control, exposure & risk assessment |
| Paginering: | Jaargang 22 (2005) nr. 2 pagina's 128-134 |
| Jaar: |
2005-02 |
| Inhoud: |
Nicarbazin and halofuginone have been widely used as coccidiostats for the prevention and treatment of coccidiosis in poultry. It has been shown that accidental cross-contamination of feed can lead to residues of these compounds in eggs and/or muscle. This paper describes a direct competitive assay for detecting halofuginone and nicarbazin, developed as qualitative screening assay. In an optimized competitive ELISA, antibodies showed 50% binding inhibition at approximately 0.08 ng ml-1 for halofuginone and 2.5 ng ml-1 for dinitrocarbanilide (marker residue for nicarbazin). Extraction from the matrix was carried out with acetonitrile followed by a wash with hexane. The assay's detection capability (CCβ) for halofuginone was <0.5 µg kg-1 in egg and <1 µg kg-1 in muscle. For dinitrocarbanilide, the CCβ was estimated at <3 µg kg-1 in egg and <10 µg kg-1 in chicken muscle. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 12 van 13 gevonden artikelen
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