Simultaneous determination of 4-hydroxy-2-alkenals, lipid peroxidation toxic products
Titel:
Simultaneous determination of 4-hydroxy-2-alkenals, lipid peroxidation toxic products
Auteur:
Surh, J. Kwon, H.
Verschenen in:
Food additives and contaminants. Pt. A, Chemistry, analysis, control, exposure & risk assessment
Paginering:
Jaargang 20 (2003) nr. 4 pagina's 325-330
Jaar:
2003-04
Inhoud:
Lipid peroxidation has been associated with cell injury and possible carcinogenecity, for the products can damage cellular membrane and DNA. Among the products formed from lipid peroxidation, 4-hydroxy-2-alkenals are the major components and have been suggested to play a role in cellular toxicity. However, the difficulties of the quantification due to their strong reactivity during laboratory procedures hinder the exposure measurement of the compounds. A novel detection method based on the conversion of 4-hydroxy-2-alkenals to stable diols and to subsequent ortho -esters has been developed. Simultaneous detection in a sensitive single-ion monitoring mode by GC/MS was carried out, monitoring m/z 129, which was the common fragment ion for the ortho -esters derived from 4-hydroxy-2-alkenals. The proposed method was used to quantify 4-hydroxy-2-nonenal in oils and in oxidized arachidonic acid.
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