Production of aflatoxins B1, B2, G1, and G2 in pure and mixed cultures of Aspergillus parasiticus and Aspergillus flavus
Titel:
Production of aflatoxins B1, B2, G1, and G2 in pure and mixed cultures of Aspergillus parasiticus and Aspergillus flavus
Auteur:
Wilson, D. M. King, J. K.
Verschenen in:
Food additives and contaminants. Pt. A, Chemistry, analysis, control, exposure & risk assessment
Paginering:
Jaargang 12 (1995) nr. 3 pagina's 521-525
Jaar:
1995-05
Inhoud:
Production of aflatoxins B1, B2, G1, and G2 in pure and mixed cultures of Aspergillus parasiticus and aspergillus flavus were compared. Differing percentages of A. parasiticus (NRRL 2999) and A. flavus (NRRL 5520) conidia were used as inoculum and allowed to grow in static liquid culture for 10 days. The pure and mixed cultures of A. parasiticus/A. flavus were extracted and the aflatoxins partially purified. High-performance liquid chromatography was then used to determine aflatoxin concentrations. The maximal decreases of G1 and G2production occurred when the inocula went from 0 to 12.5% or 25% A. flavus. Aflatoxins G1and G2 comprised less than 10% of the total aflatoxins when the A. parasiticuslA. flavus inocula contained greater than 25% A. flavus. Production of aflatoxins B1 and B2 increased slightly as the percentage of A. flavus in the A. parasiticuslA. flavus mixed inoculum increased. A. flavus is apparently capable of suppressing accumulation of aflatoxins G1 and G2 by A. parasiticus when these fungi are grown in mixed culture.
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