Characterization of the principal colouring components of annatto using high performance liquid chromatography with photodiode-array detection
Titel:
Characterization of the principal colouring components of annatto using high performance liquid chromatography with photodiode-array detection
Auteur:
Scotter, M. J. Thorpe, S. A. Reynolds, S. L. Wilson, L. A. Strutt, P. R.
Verschenen in:
Food additives and contaminants. Pt. A, Chemistry, analysis, control, exposure & risk assessment
Paginering:
Jaargang 11 (1994) nr. 3 pagina's 301-315
Jaar:
1994-05
Inhoud:
The principal colouring components of the natural food colouring material annatto, the 9'-cis- and all-trans- isomers of bixin and norbixin, have been prepared in pure form. A reverse-phase HPLC method utilizing photodiode-array detection has been developed to enable their Chromatographic and spectroscopic characterization. One minor component, a di-cis-isomer of norbixin has also been identified and characterized.