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| Titel: |
Desorption dynamics of neutral molecules in matrix-assisted laser desorption/ionization |
| Auteur: |
Tsai, S. -T. Chen, C. -H. Lee, Y. T. Wang, Y. -S. |
| Verschenen in: |
Molecular physics |
| Paginering: | Jaargang 106 (2008) nr. 2-4 pagina's 239-247 |
| Jaar: |
2008-01 |
| Inhoud: |
This work studies the expansion dynamics of molecules ejected from sample crystals in matrix-assisted laser desorption/ionization (MALDI) reaction. Measurements were made in two steps-sample desorption using a 355 nm pulsed laser beam and gas-phase postionization using a 266 nm laser at various delays. The estimated ion production yield is approximately 104 times better than that obtained using conventional MALDI methods. A model system of co-crystallized tryptophan (Trp) analyte and 2,4,6-trihydroxyacetophenone (THAP) matrix yielded overlapping time-of-arrival profiles. This result suggests that translational behaviours of gas-phase Trp and THAP are thermalized during expansion. The observed plume velocity depends strongly on the matrix and analyte composition: the most probable velocity is 214 m/s for pure Trp and 450 m/s for pure THAP. The incorporation of Trp into THAP makes both their velocities fall between the two extreme cases. Multiple half-space Maxwellian curves adequately describe the observed desorption velocity profile, implying that MALDI consists of multiple desorption components. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 7 van 38 gevonden artikelen
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