Enzyme activity, nitrogen uptake and corn growth as affected by ammonium concentration in soil solution
Title:
Enzyme activity, nitrogen uptake and corn growth as affected by ammonium concentration in soil solution
Author:
Anghinoni, I. Magalhaes, J. R. Barber, S. A.
Appeared in:
Journal of plant nutrition
Paging:
Volume 11 (1988) nr. 2 pages 131-144
Year:
1988-02
Contents:
Few studies have related the activity of enzymes responsible for nitrogen incorporation in the plant with ammonium concentration in the soil. The objective of this experiment was to relate the glutamine synthetase and glutamate dehydrogenase activities, nitrogen uptake and corn (Zea mays L.) growth with ammonium concentration in soil solution. Corn, cv. Beck 65X, was grown for 20 days after seeding in Raub silt loam (Aquic Argiudoll) in a climate controlled chamber. Rates of 0, 90, 180, 450, 900, 1800 and 4500 mg N.kg-1 of soil provided six concentrations in fertilized soil equivalent to 90 mg N.kg-1 of soil applied to 100, 50, 20, 10, 5 and 2% of the soil volume. The soil was previously leached to remove nitrate and an inhibitor of nitrification (Nitrapyrin) was used. High rates of nitrogen induced very high ammonium concentrations in soil solution and limited plant growth. While glutamine synthetase activity increased with ammonium concentration up to 3mM and then decreased, glutamate dehydrogenase activity increased up to 1.3 mM ammonium and remained fairly constant. There was a direct relation of glutamine synthetase activity in the root tissue with nitrogen uptake and corn growth. Glutamine synthetase played an important role in nitrogen incorporation into the plant. Soil solution concentrations of ammonium higher than 3 mM were toxic for corn growth in Raub soil.