The in vitro metabolism of lindane by an enzyme preparation from chicken liver
Titel:
The in vitro metabolism of lindane by an enzyme preparation from chicken liver
Auteur:
Foster, T. S. Sana, J. G.
Verschenen in:
Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes
Paginering:
Jaargang 13 (1978) nr. 1 pagina's 25-45
Jaar:
1978
Inhoud:
The soluble fraction (105,000 x g, 30 min) of chicken liver homogenates contained an enzyme(s), probably a dehydrochlorinase(s), which metabolized lindane in vitro. The reaction was glutathione dependent and took place anaerobically. The enzyme (s) also meta-bolized the α- and δ-isomers but not the β-isomer. About 66% of the in vitro metabolites were soluble in petroleum ether. From the ether-soluble fraction, o-, p- and m-dichlorobenzene, 1,2,3- and 1,2,4-trichlorobenzene, γ-2,3,4,5,6-pentachlorocyclohex-l-ene (y-PCCH), 2,3- and 2,4-dichlorophenol and 2,4,6-trichlorophenol were identified by comparison of mass spectra and gas chromatographic data with those of reference compounds. Seven additional metabolites were tentatively identified from their mass spectra data. These metabolites were: chlorobenzene, 2 isomers of trichlorocyclohexene, dichloro-cyclohexadienetriol, chlorophenol, trichlorocyclohexenol and trichlorocyclohexanediol. The petroleum ether-extracted aqueous phase contained a number of unidentified conjugated metabolites. It was concluded that the metabolic pathway for lindane in the chicken is quite similar to that in the pheasant.
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