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Details van artikel 4 van 16 gevonden artikelen
| Titel: |
Development of a fully suspended, multi-stage bioreactor system for trichloroethylene degradation based on soluble monooxygenase cometabolism |
| Auteur: |
Tschantz, Michael F. Bowman, John P. Evans, Frederick Bienkowski, Paul R. Donaldson, Terry L. Sayler, Gary S. |
| Verschenen in: |
Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering |
| Paginering: | Jaargang 31 (1996) nr. 1 pagina's 249-266 |
| Jaar: |
1996-01 |
| Inhoud: |
The design and operation of a pressurized dual-staged continuous flow bioreactor for the biodegradation of trichloroethylene (TCE) is presented. The reactor was designed to maximize the utility of the enzyme soluble methane monooxygenase (sMMO) by the methanotroph, Methylosinus trichosporium OB3b (PP358), which can produce sMMO even in the presence of copper. Methane-fed cell growth in a continuous stirred tank reactor (CSTR) was decoupled from TCE oxidation in a methane-free series of plug-flow columns, minimizing competitive inhibition. The biomass concentrations were maximized by operating the reactor at 3.04 atm, increasing the gas-phase solubility of methane, which increased the growth rate of the cells. Furthermore, washout of the reactor biomass in the continuous flow-through system was minimized through the use of a tangential flow dewatering device. The biodegradative potential of the reactor was demonstrated by feeding TCE at concentrations of 0.2 mg/L and 1 mg/L at 2 mL/min. The TCE was completely degraded when TCE was fed at 0.2 mg/L and over 95% of the TCE fed at 1 mg/L was degraded. The measured TCE losses were due entirely to biodegradation. Batch abiotic experiments showed the reactor held pressure, and losses due to stripping and adsorption were minimal over 4 h. The TCE was fed abiotically into the reactor at 1 mg/L, and 92 h of operation was necessary for the reactor TCE concentrations to accumulate to that of the feed. The abiotic mass balances demonstrated that in the absence of biodegradation, the reactor TCE concentrations would accumulate to that of the feed. The ability to close mass balances, coupled with the ability to closely control and monitor reactor conditions, allows for a system that may be utilized to construct a mechanistic model describing the kinetics of TCE degradation by sMMO and may potentially maximize the utility of the methanotrophs for TCE biodegradation at high feed concentrations. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 16 gevonden artikelen
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